UNI er oppdelt i 4 regioner;

• UNI Europa (Brussels) - 50 land, 320 fagforeninger og 7 millioner medlemmer
• UNI Africa (Abidjan/Johannesburg)
• UNI Americas (Montevideo)
• UNI Asia & Pacific (Singapore)

Organisasjonen er også inndelt i 13 ulike sektorer og 4 grupper:

• Cleaning/security
• Commerce
• Finance
• Gaming
• Graphical & Packaging
• Hair & Beauty
• ICTS (Information and Communication Technology Services)
• MEI (Media Entertainment & Arts)
• Plattform & Agency workers
• Post & Logistics
• Tourism
• UNICARE (the private care sector and social insurance industry)
• World Players

4 grupper:

• UNI Equal Opportunities
• UNI Professionals and Managers (UNI P&M) 
• UNI SCORE (Strategic Campaigns, Organising, Research and Education) 
• UNI Youth  

Leder i Negotia Ung, Trond Vegard Sæle, er president i UNI Youth Europa(2019-2021), samt president i UNI Youth World (2019-2020). 1. nestleder Eirik Bornø er 2. vara til UNI World Executive Board.